The RecombiLISA TSH ELISA Kit is a solid phase enzyme linked immunosorbent assay for the quantitative determination of the levels of Thyroid Stimulating Hormone (TSH) in human serum.
TSH ELISA Kit
- Sensitivity: Detects TSH levels as low as 0.035 µIU/mL
- Specificity: No cross reactivity detected with related hormones: FSH, LH and hCG
- Linearity: 1:128 dilution on 36 ?IU/mL sample
- Precision: intra assay precision: CV 7.5%; inter assay precision: CV 8.0 %
- Accuracy: R2= 0.986 against Roche TSH Chemiluminescence Immunoassay
- Hook effect: No hook effect up to 8000 ?IU/mL
- Interference: not affected by Hemoglobin 500mg/dL, Diltiazem 15uM, Bilirubin 20 mg/dL, Erythromycin 81.6uM, Triglycerides 3g/dL, Verapamil 4.4uM, HAMA 52.5ng/mL, T3 100ng/mL, RF 215IU/mL, T4 100ug/dL, Acetaminophen 1324uM
- Calibration: Human TSH WHO 3rd International Standards (81/565 NIBSC)
- Calibrators: 0, 0.5, 2.0, 5.0, 10, 20, 40 µIU/mL in biological matrix
- Shelf life: 18 months
- Assay time: 80 minutes
- Microwells coated with anti-TSH Ab 1 plate, 96 wells
- TSH Standards: S1 (0 ?IU/mL) 1 mL
- TSH Standards: S2 (0.5 ?IU/mL) 1 mL
- TSH Standards: S3 (2 ?IU/mL) 1 mL
- TSH Standards: S4 (5 ?IU/mL) 1 mL
- TSH Standards: S5 (10 ?IU/mL) 1 mL
- TSH Standards: S6 (20 ?IU/mL) 1 mL
- HRP-anti-TSH conjugates 6 mL
- Substrate 12.0 mL TME2000
- Concentrated wash buffer (40X) 25 mL
- Stop solution 7.5 mL
- Product insert 1 set
